Journal: BMC cancer

Article Title: Analysis of cells of epithelial, connective tissue and immune differentiation in HPV-positive-, HPV-negative oropharyngeal carcinoma and normal oropharyngeal tissue by immunofluorescence multiplex image cytometry: a preliminary report.

doi: 10.1186/s12885-023-11440-x

Figure Lengend Snippet: Fig. 3 Histograms of positive- and isotype controls, and patients with oropharyngeal squamous cell carcinoma positive controls. Comparison of histograms of fluorescence signals between positive controls, patients with oropharyngeal squamous cell carcinoma (OPSCC) and isotype controls. Histograms of fluorescence signals after control incubation in the middle histograms (isotype controls) and specific fluorescence signals after test incu bation in the upper (positive controls) and lower (patients with OPSCC) histograms. Presentation of a random cell sample of the positive controls, their isotype controls and the patients with OPSCC. X-axis: mean intensity in logarithmic scale. Y-axis: Cell count. The left histograms represent cytokeratin fluorescence signal of CAL-27 tumor cell line, its isotype and patients with OPSCC, the middle histograms vimentin fluorescence signal of human gingiva fibroblast cell line, its isotype and patients with OPSCC and the right histograms CD45/CD18 fluorescence signal of lymphoma tissue, its isotype and patients with OPSCC

Article Snippet: A human gingival fibroblast (hGF) cell line (CLS order number: 300,703; CLS Cell Lines Service, Eppelheim, Germany), cultivated in DMEM/F12 medium, served as positive control for vimentin [27].

Techniques: Comparison, Fluorescence, Control, Incubation, Cell Characterization

Journal: BMC Microbiology

Article Title: First human cell-based cultivation system for the syphilis spirochete Treponema pallidum

doi: 10.1186/s12866-026-04856-5

Figure Lengend Snippet: Human foreskin fibroblast cell lines support the growth of T. pallidum in vitro . A Human foreskin fibroblast cell lines (MoNa, HFF1, and HFFC) support the growth of T. pallidum in long-term, continuous, in vitro cultivation. While cultivation of strain SS14 was discontinued after 10 weeks, in vitro cultures of the DAL-1 strain have been ongoing for over one year. During this period, scheme of subcultures was optimized (up to 1:20). A booster passage (*), extending the standard 7-day subculture to a 14-day period with a cultivation medium exchange on day 7, was employed to enrich the treponemal culture during critical declines. In vitro cultivation was performed in triplicate (i.e., in three cultivation wells) and the mean values for each subculture are presented in graph. Source data from individual wells are shown in Supplementary Table . B Parallel cultivation for seven days ( n = 15) with defined DAL-1 inoculum (10 6 treponemes) validated the differences in growth support of individual foreskin cell lines. Treponemal growth on human foreskin fibroblast cells was slower compared to growth on rabbit epithelial Sf1Ep cells. Red bar, mean. C Human foreskin-based cultivation system supports the growth of various T. pallidum strains ( n = 8), from the Nichols-like as well as the SS14-like cluster. Note that human foreskin fibroblast cells were prepared as an equal mixture of three tested cell lines. Each T. pallidum strain was cultivated in a single in vitro well, representing a sole experimental replicate used for data acquisition

Article Snippet: Human foreskin fibroblasts HFF1 (SCRC-1041; ATCC) and HFFC (300715; Cytion) were purchased, while the third cell line (MoNa) was kindly provided by Dr. Vladimir Rotrekl (Masaryk University), and was originally obtained from the National Tissue Centre (Czech Republic).

Techniques: In Vitro

Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects

Article Title: Tunable assembly of mixed PLGA-lipid nanoparticles via monoolein with improved Reactive Oxygen Species cell protection

doi: 10.1016/j.colsurfa.2025.136864

Figure Lengend Snippet: Fig. 9. ROS Scavenging in human dermal fibroblasts (HDF). (A) Cytotoxicity of IDE alone, H-MO-DNP-IDE-10 and L-MO-DNP-IDE-10 in HDF cell culture after 24 h of incubation. (B) Hystogram showing the % ROS Positive cells after treatment with IDE alone, H-MO-DNP-IDE-10 and L-MO-DNP-IDE-10 for 3 h followed by 1 mM H2O2 treatment for 1 h. ROS levels were measured by flow cytometry analyses with the DCFH-DA dye (FL1 channel, Ex: 488 nm/Em: 519 nm). Results are reported by using histogram subtraction statistics tool in FCS 7 Express with untreated cells as control (% Positive cells with respect to control). (C) Representative flow cytometric images of HDF in the conditions tested. The number of cells is plotted versus the DCFH fluorescence detected by the FL-1 channel. *P < 0.05.

Article Snippet: Human Dermal Fibroblast (HDF) cells (CLS Cell Lines Service GmbH, No. 300606) were obtained from American Type Culture Collection (ATCC) Manassas, Virginia, USA.

Techniques: Cell Culture, Incubation, Flow Cytometry, Control, Fluorescence

Journal: Pharmaceuticals

Article Title: Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation

doi: 10.3390/ph15020254

Figure Lengend Snippet: Anti-aging activities’ evaluation of fenugreek extract: ( a ) In vitro collagenase inhibition of fenugreek extract (denoted as Extract) and liponiosome encapsulating fenugreek extract (denoted as LNF). ( b ) Effect of fenugreek extract on collagen production. Human dermal fibroblast cells were treated with 125 µg/mL of fenugreek extract and rutin, and 50 µg/mL of vitamin C as a positive control and 0.005% DMSO as a control (vehicle), for 7 and 14 days. Data are reported as means ± SD ( n = 3). *, p < 0.05; ***, p < 0.001.

Article Snippet: Human dermal fibroblast cells were purchased from the American Type Culture Collection: ATCC (PCS-201-010) and immortalized human keratinocytes (HaCaT) were purchased from Cell Lines Service, Germany (Cat. No. 300493).

Techniques: In Vitro, Inhibition, Positive Control, Control

Journal: Pharmaceuticals

Article Title: Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation

doi: 10.3390/ph15020254

Figure Lengend Snippet: Evaluation of LNF activities: ( a ) Cytotoxicity induced by LNF: The effect of blank, LNF, and fenugreek extract on cell viability. Human dermal fibroblast cells were treated with different concentrations of blank (liponiosome without fenugreek extract denoted as Blank), LNF, and fenugreek extract for 24 h. Data are means ± SD ( n = 3). ( b ) Collagen production induced by LNF: The effect of LNF on collagen production. Human dermal fibroblast cells were treated with 0.005% DMSO as a control (vehicle), 7 µg/mL of extract, and 100 µg/mL of LNF (7 µg/mL of extract equivalence), together with blank particles, for 7 and 14 days. Data are represented as means ± SD ( n = 3). *, p < 0.05.

Article Snippet: Human dermal fibroblast cells were purchased from the American Type Culture Collection: ATCC (PCS-201-010) and immortalized human keratinocytes (HaCaT) were purchased from Cell Lines Service, Germany (Cat. No. 300493).

Techniques: Control

Journal: Pharmaceuticals

Article Title: Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation

doi: 10.3390/ph15020254

Figure Lengend Snippet: Inhibition of UV-induced MMPs and interleukin secretion on co-cultured skin cells by fenugreek extract and LNF: ( a ) Effect of UV-induced cytotoxicity after the pretreatments of resveratrol, rutin, fenugreek extract, blank nanoparticles (liponiosome without fenugreek extract), LNF nanoparticles, and 0.005% DMSO as a control (vehicle). HaCAT and human dermal fibroblast cells were co-cultured and pretreated with 7 µg/mL of extract and 100 µg/mL of LNF (7 µg/mL of extract equivalence), together with 100 µg/mL of blank, 10 µg/mL of resveratrol, 7 µg/mL of rutin as a positive, and 0.005% of DMSO as a control (vehicle), for 24 h before UV exposure. Data are means ± SD ( n = 3). *, p < 0.05. ( b ) The levels of UV-induced MMP1 and MMP9 secretions after fenugreek extract and LNF treatments. Data are reported as means ± SD ( n = 3). *, p < 0.05. ( c ) The levels of UV-induced IL-6 and IL-8 secretions after fenugreek extract and LNF treatments. Data are reported as means ± SD ( n = 3). *, p < 0.05.

Article Snippet: Human dermal fibroblast cells were purchased from the American Type Culture Collection: ATCC (PCS-201-010) and immortalized human keratinocytes (HaCaT) were purchased from Cell Lines Service, Germany (Cat. No. 300493).

Techniques: Inhibition, Cell Culture, Control

Journal: Cells

Article Title: Accelerated Aging Effects Observed In Vitro after an Exposure to Gamma-Rays Delivered at Very Low and Continuous Dose-Rate Equivalent to 1–5 Weeks in International Space Station

doi: 10.3390/cells13201703

Figure Lengend Snippet: Major features of the cell lines used in this study.

Article Snippet: EROS01F , Skin fibroblast , COPERNIC.

Techniques:

Journal: Cells

Article Title: Accelerated Aging Effects Observed In Vitro after an Exposure to Gamma-Rays Delivered at Very Low and Continuous Dose-Rate Equivalent to 1–5 Weeks in International Space Station

doi: 10.3390/cells13201703

Figure Lengend Snippet: Fitting data of the number of γH2AX foci as a function of dose.

Article Snippet: EROS01F , Skin fibroblast , COPERNIC.

Techniques:

Journal: Cells

Article Title: Accelerated Aging Effects Observed In Vitro after an Exposure to Gamma-Rays Delivered at Very Low and Continuous Dose-Rate Equivalent to 1–5 Weeks in International Space Station

doi: 10.3390/cells13201703

Figure Lengend Snippet: Percentage of ATM crowns and of highly damaged cells (HDC) observed in the indicated cell lines at each week of exposure to 120 mSv/y (( A ): radioresistant skin fibroblasts; ( B ): radiosensitive skin fibroblasts; ( C ): lens cells; ( D ): bone cells). Each plot corresponds to the mean of three replicated ± SEM. Each week represents an exposure to 2.3 mSv.

Article Snippet: EROS01F , Skin fibroblast , COPERNIC.

Techniques:

Journal: bioRxiv

Article Title: CRISPR-pass: Gene rescue of nonsense mutations using adenine base editors

doi: 10.1101/545723

Figure Lengend Snippet: Restoring abbreviated XPC gene expression in patient-derived fibroblasts. (a) Scheme for ABE-induced read-through of an XPC-associated PTC. (b) Targeted deep sequencing data showing the A-to-G substitution rate induced by ABEmax treatment at the PTC site in the XPC gene. (c) Expression level of the XPC protein in XPC mutant cells rescued by treatment with ABEs (ABEmax or xABE), compared with the expression level in untreated cells and cells treated with ataluren or gentamicin for 48 h. (d) Cell viability of WT skin fibroblasts (BJ-5ta), XPC mutant cells (GM14867), and XPC mutant cells treated with ABEs (ABEmax or xABE), ataluren, or gentamicin at 3 days after exposure to 254 nm ultraviolet radiation at a dose of 25 J/m 2 . P -values were calculated by one-way ANOVA with post-hoc Bonferroni’s multiple comparison tests ( n = 6). P- values indicators from a comparison with GM14867 cell viability are shown above each treatment group. NS , not significant ( P > 0.05); *, P < 0.05; ***, P < 0.001. (e) Prolonged expression of the XPC protein after CRISPR-pass treatment. Significant and stable XPC protein expression was observed until at least 4 weeks after ABEmax treatment. However, XPC protein expression declined after removal of ataluren and gentamycin. Proteins were also prepared from ABEmax-treated XPC mutant cells at 2 and 4 wk (subculturing twice per week) for comparison. Blue and red arrowheads indicate the positions of XPC protein.

Article Snippet: GM14867 ( XPC mutant fibroblasts) were purchased from Coriell Institute and maintained in Eagle's Minimum Essential Medium (EMEM) with 15% FBS and a penicillin/streptomycin mix.

Techniques: Expressing, Derivative Assay, Sequencing, Mutagenesis, CRISPR, Subculturing Assay